Integrating Histology And Mri On The First Digital Brain...

Integrating histology and MRI in the first digital brain of common squirrel monkey, Saimiri sciureus Peizhen Suna, Prasanna Parvathanenia, Kurt G. Schillingb,c, Yurui Gaob,c, Vaibhav Janve b,c, Adam Andersonb,c, Bennett A. Landman*,a,b,c,d aElectrical Engineering, Vanderbilt University, Nashville, TN USA, bBiomedical Engineering, Vanderbilt University, Nashville, TN USA, cInstitute of Image Science, Vanderbilt University, Nashville, TN USA, dComputer Science, Vanderbilt University, Nashville, TN USA. ABSTRACT This effort is a continuation of development of a digital brain atlas of the common squirrel monkey, Saimiri sciureus, a New World monkey with functional and microstructural organization of central nervous system similar to that of†¦show more content†¦We describe mapping of histology and MRI based data of the common squirrel monkey and construction of a viewing tool that enable online viewing of these datasets. The previously descried atlas MRI is used for its deformation to provide accurate conformation to the MRI, thus adding information at the histological level to the MRI volume. This paper presents the mapping of single 2D image slice in block face as a proof of concept and this can be extended to map the atlas space in 3D coordinate system as part of the future work and can be loaded to an XNAT system for further use. Keywords: primate neuroanatomy, atlas, magnetic resonance imaging, diffusion tensor imaging 1. INTRODUCTION Atlases provide a central coordinate system in which to merge information from different imaging modalities, across time within a single subject, between subjects, and among studies. Digital atlases have come into common use from brain mapping with 3-D imaging techniques, e.g., magnetic resonance imaging (MRI). Integration of histology images with typical multi-modal MRI atlases (e.g., for the squirrel monkey [1]) is greatly complicated by the fact that histological sections are, by their nature, extraordinarily isotopic. Any out-of-plane rotation/resampling would result substantive partial volume artifacts. During the sectioning process, it is commonly possible to acquire photographic images of the block face